Expression Database

GUDMAP:6953

Gene

Hoxa3 homeobox A3

Theiler Stage

Images





	Tunica albuginea and rete testis are positive.
	Tunica albuginea and rete testis are positive.
	Tunica albuginea and rete testis are positive.

Submitters

Principal Investigator:	Kevin Gaido, The Hamner Institutes of Health Sciences, 6 Davis Dr, Research Triangle Park, gaido@thehamner.org
Authors:	Helen Shi, Kamin Johnson, Kevin Gaido
Submitted By:	Janan Hensley, The Hamner Institutes of Health Sciences, 6 Davis Dr, Research Triangle Park, jhensley@thehamner.org
Archive ID:	39

Expression Mapping

Expression Strengths Key:

	Present (unspecified strength)
	Present (strong)
	Present (moderate)
	Present (weak)
	Uncertain
	Not Detected

Expression Patterns Key:

	Graded
	Regional
	Spotted
	Ubiquitous
	Restricted
	Single cell
	Contains note

View annotated components as a list

Hide annotation under groups

Javascript Tree Menu G Group or group descendent. Groups provide alternative groupings of terms.

Probe

Probe Name:

MGI:1201986

Clone Name:

Hoxa3 cDNA1

Sequence:

Fully Sequenced. Probe sequence spans from 684 to 1172 of NM_010452.2

Gene:

Symbol: Hoxa3

Name: homeobox A3 (MGI:96175)

5' primer sequence:

TGTCTCTCCCCCTCAAAGTG

3' primer sequence:

ACACTGTTGACCAGCGAATG

5' primer location:

684

3' primer location:

1172

Origin of Clone used to make the Probe:

cDNA		Strain:
		Tissue:	testis

Probe Type:

RNA

Type:

antisense

Labelled with:

digoxigenin

Visualisation method:

alkaline phosphatase + NBT/BCIP

Author Notes:

The template DNA sequence was PCR amplified from pool of various gestation ages of CD1 mouse testis cDNA using: 5' primer: TGTCTCTCCCCCTCAAAGTG, 3' primer: ACACTGTTGACCAGCGAATG. T7 is incorporated in the transcription step using DIG RNA Labeling kit.

Specimen

Theiler Stage:

TS24

Other Staging System:

Strain:

CD1

Sex:

male

Genotype:

Wild Type

Specimen Preparation:

Section

	Fixation Method:	4% paraformaldehyde
	Embedding:	cryosection

Experiment Notes:

Linked Publications

Linked Submissions

Acknowledgements